Enzyme Immunoassay

Binding Site EIA plates are coated with a specific protein (e.g. antibody or antigen). Calibrator, control or patient samples are added to the pre-coated well which binds to the specific protein present on the plate. This reaction is detected and quantified using an antibody linked to an enzyme. Following the addition of the enzyme substrate, a color reaction will occur. The concentration of the substance in the sample is measured by spectrophotometry comparing to known calibrators.

• EIA is a very sensitive technique which can be run manually or with automated EIA systems; results are typically available within 2 hours
• 96 break-apart wells offer a solution for smaller workloads
• Our vaccine response EIA kits are useful tools in assessing the function of the immune system (e.g. VaccZyme Diphtheria toxoid kit)
• The accurate measurement of specific antibodies is also important during the manufacture of therapeutic immunoglobulins and hyperimmune products (e.g. VaccZyme Tetanus toxoid kit)