Hevylite®

The Hevylite assays individually measure IgGκ, IgGλ, IgAκ, IgAλ, IgMκ and IgMλ in g/L.

These values can be used in different ways to assess a patient with a monoclonal gammopathy:

Term	Definition	Description	Example in an IgGk MM patient
HLC	Heavy and light chain isotypes
iHLC	Involved HLC	The HLC type produced by the tumour. Measures monoclonal production	IgGκ
uHLC	Uninvolved HLC	Measures polyclonal production	IgGλ
HLC ratio	e.g. IgAκ/IgAλ	Indicates clonality	IgGκ/IgGλ
dHLC	iHLC - uHLC	For a particular immunoglobulin isotype, the difference in concentration between the iHLC and uHLC	IgGκ-IgGλ
HLC-pair suppression	Only uHLC is below normal reference interval	The ratio must be abnormal	Suppression of IgGλ

Normal ranges for Hevylite values in serum are:

Normal adult serum	95 percentile range for SPAPLUS
IgG Kappa	3.84 - 12.07 (g/L)
IgG Lambda	1.91 - 6.74 (g/L)
IgGκ/IgGλ ratio	1.12 - 3.21
IgA Kappa	0.57 - 2.08 (g/L)
IgA Lambda	0.44 - 2.04 (g/L)
IgAκ/IgAλ ratio	0.78 - 1.94
IgM Kappa	0.19 - 1.63 (g/L)
IgM Lambda	0.12 - 1.01 (g/L)
IgMκ/IgMλ ratio	1.18 - 2.74